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Updated: May 30, 2026
13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis
Published on: March 22, 2024
[Studies on the chemical constituents of Paris bashanensis]
Zhi-Gang Zhang1, De Zhao, Jun Deng
1College of Pharmaceutical Sciences, Southwest University, ChongQing 400715, China. zzgbest007@163.com
Objective:
Study the chemical constituents of the rhizomes of Paris bashanensis to search after the alternative resourc for the Chinese medicinal material Rhizoma Paridis.
Methods:
The n-BuOH extracts of P. bashanensis was applied to silica gel column and eluted with EtOAc-EtOH,then the gained fractions were further purified by chromatography on Sephadex LH-20 column and PreRP-HPLC to give pure compounds whose structures were elucidated mainly on the basis of analyzing the spectral data of MS,1H-NMR, 13C-NMR,2D-NMR.
Results:
Five compounds were isolated and identified as P-ecdysone (1), pinnatasterone(2), pennogenin-3-0-alpha-L-rhamnopyranosyl (1-->2)-[alpha-L-arabinofuranosyl ( 1- -4) ] -pf-D-glycopyranoside (3), diosgenin-3-O-alpha-L-rhamnopyranosyl (1-->2) - [ a-L-arabinofuranosyl (1-->4)]-beta-D-glycopyranoside(4), pennogenin-3-O-alpha-L-rhamnopyranosyl (1-->4) -a-L-rhamnopyranosyl (1-4)-[alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glycopyranoside (5).
Conclusion:
Compound 1-5 are obtained from this plant for the first time.
