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Published on: April 23, 2019
Analytical aspects in doping control: challenges and perspectives.
Flavia Badoud1, Davy Guillarme, Julien Boccard
1School of Pharmaceutical Sciences, University of Geneva and Lausanne, 20 Bd d'Yvoy, 1211 Geneva 4, Switzerland.
Forensic Science International
|August 10, 2011
Summary
Analytical methods in anti-doping are evolving, focusing on enhanced sensitivity, selectivity, and speed. Advanced techniques like mass spectrometry and chromatography are crucial for accurate detection of prohibited substances and metabolites in athletes.
Area of Science:
- Analytical Chemistry
- Sports Science
- Biochemistry
Background:
- Anti-doping analysis has faced persistent analytical challenges since the 1960s.
- Increasing sensitivity, selectivity, and speed are critical for modern doping control.
Purpose of the Study:
- To discuss the evolution of analytical processes in doping control.
- To highlight advancements in separation techniques and mass spectrometry.
- To explore the analysis of proteins, peptides, and indirect anti-doping approaches.
Main Methods:
- Gas chromatography (GC) and liquid chromatography (LC) for separation.
- Mass spectrometry (MS) with accurate mass determination for identification.
- Analysis of proteins, peptides, and indirect biological response monitoring.
Main Results:
- Improved sensitivity and selectivity in detecting prohibited substances and metabolites.
- Enhanced structural identification using elemental composition and isotope patterns.
- Development of reliable methods to distinguish between negative and suspicious samples.
Conclusions:
- Advanced analytical techniques are essential for accurate and reliable anti-doping strategies.
- Continued innovation is needed to address emerging challenges, including peptide analysis.
- Indirect approaches offer a holistic view of doping agent effects.
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