Preliminary study for simultaneous detection and quantification of androgenic anabolic steroids using ELISA and...

Area of Science:

• * Analytical Chemistry
• * Biochemistry
• * Forensic Science

Background:

• * Accurate detection of anabolic androgenic steroids (AAS) is crucial for doping control and clinical diagnostics.
• * Existing methods often struggle with simultaneous detection of multiple AAS or are affected by cross-reactivity.
• * Development of a sensitive and specific multidetection assay for AAS is needed.

Purpose of the Study:

• * To develop and validate a multidetection method for identifying and quantifying anabolic androgenic steroids (AAS).
• * To combine multiple competitive enzyme-linked immunosorbent assay (ELISA) formats with advanced data analysis for enhanced specificity.
• * To establish a reliable assay capable of simultaneous detection and concentration prediction of multiple AAS.

Main Methods:

• * Utilized multiple competitive enzyme-linked immunosorbent assay (ELISA) kits with distinct cross-reactivity profiles.
• * Applied multivariate data analysis, specifically principal component analysis (PCA).
• * Implemented a novel K-nearest line classifier for data interpretation and steroid identification.

Main Results:

• * Achieved simultaneous detection of up to four different anabolic androgenic steroids.
• * Demonstrated accurate detection within a concentration range of 0.1 to 316.2 nM.
• * Reported a total correct detection rate of 90.6%, robust even in the presence of cross-reactivities.
• * Developed a concentration prediction methodology yielding satisfactory results.

Conclusions:

• * The proposed method offers a significant advancement in the multidetection, identification, and quantification of AAS.
• * The combination of competitive ELISAs and multivariate analysis provides a powerful tool for complex steroid mixture analysis.
• * This approach shows promise for applications in sports doping control and clinical monitoring requiring simultaneous AAS detection.