Related Experiment Video
Updated: May 30, 2026

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
Published on: April 1, 2013
Effect of polymer containing a naphthyl-alanine derivative on the separation selectivity for aromatic compounds in
Tadashi Nishio1, Ryosuke Kanazashi, Akari Nojima
1Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
Abstract:
A novel polymer-grafted stationary phase of high-performance liquid chromatography (HPLC) was developed, utilizing a temperature-responsive polymer containing an aromatic moiety. Firstly, we synthesized novel functional polymer poly(N-isopropylacrylamide-co-N-acryloyl-3-(2-naphthyl)-L-alanine methyl ester) [poly(NIPAAm-co-Nap)], which has temperature-responsiveness and selective retention of aromatic compounds by an intermolecular π-π interaction. The polymer exhibited a significant reversible phase transition from hydrophilic to hydrophobic in the vicinity of its lower critical solution temperature. Employing the developed polymer-grafted silica column, temperature-responsive chromatography was conducted using water as a sole mobile phase. A comparison with a conventional ODS column or a homogeneous PNIPAAm-grafted silica column showed that the retention of aromatic compounds was dramatically increased on the poly(NIPAAm-co-Nap)-grafted stationary phase. Introducing the naphthyl-alanine derivative caused a significant effect on the retention selectivity for aromatic compounds.
More Related Videos
10:53Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
Published on: October 10, 2016
11:16Preparation of Silver-Palladium Alloyed Nanoparticles for Plasmonic Catalysis under Visible-Light Illumination
Published on: August 18, 2020
Related Concept Videos
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Analyte Adsorption and Distribution
Basicity of Aromatic Amines
Polymer Classification: Stereospecificity
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...