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Effect of linoleic acid hydroperoxide on adenovirus DNA replication
Y Sasaguri1, H Yanagi, S Fukuda
1Department of Pathology, Kurume University School of Medicine, Japan.
Biochemistry International
|January 1, 1990
Abstract:
The effect of linoleic acid hydroperoxide on replication of adenovirus type 2 DNA in HeLa cells was examined. The level of the DNA was extremely reduced when the cells immediately after the infection with adenovirus type 2 were incubated for 24 h in the presence of 1 nmol/ml (measured by the thiobarbituric acid reaction and expressed in terms of malondialdehyde) of the hydroperoxide. The levels of the messenger RNA for adenovirus type 2 DNA polymerase and the expressed polymerase were equally reduced. The inhibition of DNA replication by lipid hydroperoxides in animal cells was discussed in relation to degenerative disorders.