Disposable luminol copolymer-based biosensor for uric acid in urine.

Area of Science:

• Analytical Chemistry
• Biosensors
• Electrochemistry

Background:

• Uric acid levels are important biomarkers for various health conditions.
• Existing methods for uric acid detection can be complex and expensive.
• Development of sensitive, disposable biosensors is crucial for point-of-care diagnostics.

Purpose of the Study:

• To develop a novel disposable electrochemiluminescent (ECL) biosensor for sensitive and selective detection of uric acid.
• To utilize a new copolymer of luminol and TMB for enhanced ECL properties.
• To validate the biosensor's performance using real-world urine samples.

Main Methods:

• Immobilization of luminol-TMB copolymer and uricase enzyme in chitosan on gold screen-printed cells.
• Fabrication of a double-layer design for the biosensor.
• Measurement of increasing ECL emission correlated with uric acid concentration.
• Comparison with a reference method for urine sample analysis.

Main Results:

• The developed biosensor demonstrated good mechanical properties and improved electroluminescent characteristics.
• A linear detection range for uric acid was established from 1.5×10⁻⁶ to 1.0×10⁻⁴ M.
• A low limit of detection (4.4×10⁻⁷ M) and acceptable precision (13.1% RSD at 1.0×10⁻⁵ M) were achieved.
• Satisfactory results were obtained when analyzing uric acid in 24-hour urine samples.

Conclusions:

• The novel ECL biosensor provides a sensitive, selective, and cost-effective method for uric acid determination.
• This disposable biosensor shows potential as a valuable alternative to conventional uric acid detection methods.
• The developed technology is suitable for practical application in clinical diagnostics and health monitoring.