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Gadd45a, Gadd45b and Gadd45g expression during mouse embryonic development.

Lilian T Kaufmann¹, Mathias S Gierl, Christof Niehrs

¹Division of Molecular Embryology, DKFZ-ZMBH Alliance, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 581, D-69120 Heidelberg, Germany.

Gene Expression Patterns : GEP

|August 17, 2011

Summary

Growth arrest and DNA damage (Gadd45) genes are expressed in specific embryonic mouse tissues. Their conserved and divergent expression patterns suggest roles in cell differentiation during development.

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Area of Science:

Developmental Biology
Molecular Biology
Genetics

Background:

Growth arrest and DNA damage (Gadd45) proteins are involved in cellular stress responses.
The roles of Gadd45 genes during embryonic development are largely unknown.

Purpose of the Study:

To comprehensively compare the expression of Gadd45a, Gadd45b, and Gadd45g during mouse embryonic development.

Main Methods:

In situ hybridization on sectioned and whole mouse embryos.

Main Results:

Gadd45a is prominently expressed in the neural tube, ganglia, and somites.
Gadd45b shows strong expression in the chorion but weak expression in the embryo proper.
Gadd45g expression resembles patterns in other vertebrates, suggesting a role in neurogenesis.

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Conserved expression of Gadd45 genes was observed during somitogenesis.

Conclusions:

Gadd45 genes exhibit conserved and divergent expression patterns in mouse embryos.
Expression domains predominantly involve areas of cell differentiation, aligning with known functions.