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Nanoshells for in vivo imaging using two-photon excitation microscopy
Liang Gao1, Tegy J Vadakkan, Vengadesan Nammalvar
1Department of Bioengineering, Rice University, Houston, TX 77005, USA. lg3@rice.edu
Nanotechnology
|August 17, 2011
Summary
Gold nanoshells engineered for near-infrared (NIR) excitation show promise for biomedical imaging. These particles enable visualization of single nanoparticles within blood vessels for enhanced in vivo imaging applications.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Optical Imaging
Background:
- Gold nanoshells offer tunable optical properties and good biocompatibility.
- Their potential as luminescent contrast agents for near-infrared (NIR) lasers is significant.
Purpose of the Study:
- To develop gold nanoshells with a plasmon resonance peak at 800 nm.
- To evaluate their utility for in vivo blood vessel imaging using two-photon excitation microscopy.
Main Methods:
- Engineering gold nanoshells with specific optical properties.
- Utilizing two-photon excitation microscopy at 750 nm for imaging.
- Assessing luminescent signals for contrast generation.
Main Results:
- Successfully developed nanoshells with 800 nm plasmon resonance.
- Achieved in vivo imaging of single gold nanoshell particles in blood vessels.
- Generated optical contrast for blood vessel structures via luminescence.
Conclusions:
- Demonstrated the feasibility of creating gold nanoshells with controlled optical characteristics.
- Confirmed the potential of single-particle-based nanoshells for in vivo imaging.
- Highlighted the utility of these nanoshells as luminescent contrast agents.
