Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preserving a Kinetically-Metastable Nanophase by Limited Calcination for High-Performance Protonic Ceramic Cells.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Image stitching for probe-based confocal laser endomicroscopy via a motion consistency constraint.

Biomedical optics express·2026
Same author

HIF1-driven TDP-43 stabilizes TRIP6 mRNA to drive angiogenesis and colorectal cancer progression under hypoxia.

Translational oncology·2026
Same author

A thalamus-brainstem attractor network drives history-biased decisions.

Nature·2026
Same author

Three-dimensional resolution enhancement of two-photon microscopy by combining point spread function engineering and multi-image deconvolution.

Optics letters·2026
Same author

Proteome-wide mapping of cysteine persulfidation in deep-sea hyperthermophilic archaea.

Extremophiles : life under extreme conditions·2026
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
09:01

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

Published on: April 16, 2017

Fluorescence ghost imaging with pseudothermal light.

Nian Tian1, Qingchun Guo, Anle Wang

  • 1Brittan Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China.

Optics Letters
|August 18, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel fluorescence ghost imaging technique using pseudothermal light, demonstrating its feasibility both theoretically and experimentally. The research discusses key factors for enhancing image quality, potentially advancing ghost imaging for biomedical uses.

More Related Videos

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Related Experiment Videos

Last Updated: May 30, 2026

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
09:01

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

Published on: April 16, 2017

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Quantum Imaging

Background:

  • Classical ghost imaging typically uses entangled photons.
  • Fluorescence imaging is crucial for biological and medical research.
  • Previous attempts at fluorescence ghost imaging faced limitations.

Purpose of the Study:

  • To extend ghost imaging principles to fluorescence imaging.
  • To demonstrate the feasibility of fluorescence ghost imaging with pseudothermal light.
  • To identify and discuss factors affecting image quality.

Main Methods:

  • Theoretical modeling of fluorescence ghost imaging.
  • Experimental implementation using pseudothermal light.
  • Analysis of image visibility and resolution parameters.

Main Results:

  • Successful theoretical and experimental demonstration of fluorescence ghost imaging with pseudothermal light.
  • Identification of critical parameters influencing image quality.
  • Established a foundation for improved fluorescence ghost imaging.

Conclusions:

  • Fluorescence ghost imaging is achievable using pseudothermal light.
  • Optimization of imaging parameters can enhance ghost imaging performance.
  • This work opens avenues for ghost imaging in biomedical applications.