[Alkaline phosphatase in mixed saliva in abdominal inflammatory diseases and gestoses]

By applying enzyme electrophoresis in the agar gel prepared on veronal-medinal buffer (pH 8.6) (the substrate was naphthol AS phosphate; the dye was fast blue B or PP), the authors first revealed mixed salivary alkaline phosphatase (AP) that differed in physicochemical properties from the known serum AP isoforms, in 63% of patients with cholecystitis or pancreatitis, in 90% of those with appendicitis, and in 55.3% of pregnant women with second- and third-degree gestoses. After treatment, the detection rate for salivary AP significantly decreased to 16.6, 40.0, and 6.4%, respectively. This noninvasive test may be useful for the additional diagnosis and monitoring of treatment for abdominal inflammatory diseases and gestational toxicosis.