Related Experiment Video
Updated: May 29, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
Published on: May 20, 2022
Exploring different inelastic projection mechanisms for electron tomography
B Goris1, S Bals, W Van den Broek
1EMAT, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. bart.goris@ua.ac.be
Abstract:
Several different projection mechanisms that all make use of inelastically scattered electrons are used for electron tomography. The advantages and the disadvantages of these methods are compared to HAADF-STEM tomography, which is considered as the standard electron tomography technique in materials science. The different inelastic setups used are energy filtered transmission electron microscopy (EFTEM), thickness mapping based on the log-ratio method and bulk plasmon mapping. We present a comparison that can be used to select the best inelastic signal for tomography, depending on different parameters such as the beam stability and nature of the sample. The appropriate signal will obviously also depend on the exact information which is requested.
Related Concept Videos
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Transmission Electron Microscopy
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Overview of Microscopy Techniques
Computed Tomography
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
