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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...

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Updated: May 29, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Clickable enzyme-linked immunosorbent assay.

Luiz A Canalle1, TuHa Vong, P Hans H M Adams

  • 1Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.

Biomacromolecules
|August 27, 2011
PubMed
Summary
This summary is machine-generated.

Click chemistry offers a cost-effective method for enzyme immobilization in diagnostic assays. A bicyclononyne (BCN) coating enabled efficient peptide immobilization for enzyme-linked immunosorbent assays (ELISA), presenting an alternative to traditional methods.

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Area of Science:

  • Bioconjugation Chemistry
  • Biomolecular Assays
  • Diagnostic Technology

Background:

  • Traditional enzyme immobilization methods for immunoassays can be costly and complex.
  • There is a need for efficient and selective immobilization techniques for diagnostic applications.
  • Click chemistry offers versatile bioconjugation strategies.

Purpose of the Study:

  • To evaluate click chemistry as a cost-effective and selective immobilization method for enzyme-linked immunosorbent assays (ELISA).
  • To compare copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC) for peptide immobilization.
  • To demonstrate the application of this method in rheumatoid arthritis (RA) diagnosis.

Main Methods:

  • Formulation of coatings with terminal alkyne or bicyclo[6.1.0]non-4-yne (BCN) handles.
  • Immobilization of a diagnostic peptide using CuAAC and SPAAC reactions.
  • Evaluation of immobilization efficiency and background noise in ELISA.

Main Results:

  • Terminal alkyne coatings resulted in high background ELISA signals due to copper catalyst use.
  • BCN-containing coatings enabled successful, low-background peptide immobilization via SPAAC.
  • The BCN-based method proved cost-effective compared to (strept)avidin-biotin systems.

Conclusions:

  • Strain-promoted click chemistry using BCN handles is a viable, cost-effective immobilization strategy for ELISA.
  • This approach offers an alternative to conventional immobilization techniques.
  • The technology is adaptable for various diagnostic tests beyond rheumatoid arthritis.