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Related Experiment Video

Updated: May 29, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

G-quadruplex-induced instability during leading-strand replication.

Judith Lopes¹, Aurèle Piazza, Rodrigo Bermejo

¹Recombinaison et Instabilité Génétique, Institut Curie Centre de Recherche, CNRS UMR3244, Université Pierre et Marie Curie, Paris Cedex 05, France.

The EMBO Journal

|August 30, 2011

Summary

This summary is machine-generated.

G-quadruplexes in yeast can impede DNA replication. Their instability during replication depends on orientation and requires the Pif1 helicase, impacting genome evolution.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

G-quadruplexes are four-stranded nucleic acid structures with poorly understood biological roles.
Replication of G-rich sequences poses challenges to genome stability.

Purpose of the Study:

Investigate the biological impact of G-quadruplexes on DNA replication in yeast.
Determine the factors influencing G-quadruplex stability during replication.

Main Methods:

Utilized the yeast Saccharomyces cerevisiae model system.
Employed the CEB1 tandem array and ARS305 replication origin.
Used Phen-DC(3) G-quadruplex ligand and Pif1 helicase deletion.
Applied two-dimensional (2D) gel electrophoresis to detect replication intermediates.

Main Results:

G-quadruplexes form in yeast and can hinder replication.
Replication instability of G-quadruplexes is orientation-dependent.
Instability requires intact G-quadruplex motifs, ARS305 activity, and is exacerbated by Pif1 absence or Phen-DC(3) treatment.
Replication stalling involves Rad51-Rad52-dependent X-shaped intermediates.

Conclusions:

G-quadruplex processing is crucial for replication fidelity.
Orientation-dependent replication challenges have implications for genome evolution.
Findings shed light on the maintenance of G-rich regions like telomeres.