Related Experiment Video
Updated: May 29, 2026

Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
Expression, purification, and initial structural characterization of nonstructural protein 2B, an integral membrane
Qiwei Huang1, Angela Shuyi Chen, Qingxin Li
1Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), Singapore 138669, Singapore.
Abstract:
Dengue virus causes serious diseases affecting people in tropical and sub-tropical regions. The nonstructural (NS) protein 2B is an integral membrane protein and important for the regulation of viral protease NS3, which is significant for virus replication. The NS2B-NS3 complex is an important drug target for treating dengue fever. However, little is known about the structure of NS2B in its entirety. Herein, we describe the expression and purification of this integral membrane protein from cell membrane and inclusion bodies of Escherichia coli cells. The initial nuclear magnetic resonance (NMR) and circular dichroism (CD) results indicate that the purified protein adopts alpha-helical structures in LMPG and TDPC micelles.
Related Concept Videos
Detergent Purification of Membrane Proteins
Leaky Scanning
