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[Establishment and primary application of sandwich ELISA method to detect tenascin-C]
Yu-cai Wang1, Dian-Zhong Zhang, Li-hong He
1Department of Orthopaedics, Tangdu Hospital, Fourth Miliatry Medical University, Xi'an 710038, China.
Aim:
To establish a sandwich method to detect tenascin-c on the basis of preparation of monoclonal antibodies (mAbs) against tenascin-C (TN-C).
Methods:
The ascites of three stains of mAbs (No. 1A8, 3H7 and 4D6) were prepared and purified. The mAbs were conjugated with HRP and paired, respectively. The recombinant TN-C was taken as standard to analyze the optimal combination between mAbs. The sera TN-C concentrations of patients with osteosarocoma and the normal persons were evaluated with the sandwich ELISA method.
Results:
Among these mAbs, the sensitivity was obtained when combined the coated 1A8 with HRP-4D6. The sera TN-C significantly higher than the normal controls.
Conclusion:
The sandwich ELISA method to detect TN-C was established successfully. The sera TN-C concentrations of patients with osteosarcoma and the normal persons were found distinct with the sandwich method.
