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Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

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One hundred anode microchannel plate ion detector.

Yi He¹, John F Poehlman, Andrew W Alexander

¹Indiana University, Bloomington, Indiana 47403, USA.

The Review of Scientific Instruments

|September 8, 2011

Summary

A novel 100-anode microchannel plate detector was developed for matrix assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry. This detector successfully eliminated anode crosstalk and provided spatial ion beam information, enabling peptide ion analysis.

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Published on: April 18, 2013

Area of Science:

Analytical Chemistry
Mass Spectrometry Instrumentation

Background:

Microchannel plate detectors are crucial for ion detection in mass spectrometry.
Existing detectors can suffer from signal interference and limited spatial resolution.

Purpose of the Study:

To develop and evaluate a 100-anode microchannel plate detector for MALDI-TOF mass spectrometry.
To assess the detector's performance in terms of signal integrity and spatial information acquisition.

Main Methods:

Construction of a 100-anode microchannel plate detector on a printed circuit board.
Integration of the detector with a homebuilt matrix assisted laser desorption ionization (MALDI) time-of-flight mass spectrometer.
Testing the detector's ability to record mass spectra and spatial ion distribution.

Main Results:

Successfully eliminated ringing and cross talk between anodes.
Recorded preliminary mass spectra of peptide ions.
Demonstrated the capability to determine spatial information of the ion beam due to the 100-anode design.
Observed a decrease in sensitivity over time due to charging, which was minimized by deflecting matrix ions.

Conclusions:

The 100-anode microchannel plate detector is a viable tool for MALDI-TOF mass spectrometry.
The detector offers improved signal integrity and spatial resolution compared to conventional designs.
Strategies for mitigating operational sensitivity loss have been identified.