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[Influence of polyelectrolytes on measles virus infectivity]
Aim:
Mechanism of virus inhibiting action against measles virus of polyelectrolytes (PE) polystyrolsulfonate (PSS) of various polymerization degree and 60 kDa molecular weight polyallylamine (PAA) was studied.
Materials And Methods:
Measles virus Leningrad-16 strain was used for the study. Virus infectious titer reduction kinetics after interaction with PSS with the degree of polymerization of 8 (PSS 8), 31, 77, 170, 360, 430 and PAA were determined by titration method with cytopathogenic effect detection in Vero continuous cell line. Circular dichroism and fluorescence spectra of viral proteins were obtained by using Zenith 200st spectrophotometer (Russian Federation) and Jasco J-810 dichrograph (Japan).
Results:
A significant decrease of measles virus infectious titers after interaction with PSS with the degree of polymerization of 8 and PAA in concentration of 30 mM was detected. Analysis of circular dichroism spectra and protein fluorescence allowed to determine the mechanism of interaction of the indicated PE with measles virus surface proteins. The secondary structure of viral proteins is damaged by hydrophobic polar frame of these PE, polyanion PSS 8 also interacts with positive charges of protein groups that leads to the formation of loops and tails that disrupt alpha-spirals.
Conclusion:
The studied PE could be considered as potential antiviral preparations, and methods of circular dichroism and protein fluorescence could be used to detect damage of viral protein secondary structure by agents of different kinds.
Insights
Polyelectrolytes like polystyrolsulfonate (PSS) and polyallylamine (PAA) show potential as measles virus inhibitors. They disrupt viral protein structure, reducing infectious titers and offering new antiviral strategies.
Area of Science:
- Virology
- Biochemistry
- Materials Science
Background:
- Measles virus poses a significant public health challenge.
- Developing effective antiviral agents against measles is crucial.
- Polyelectrolytes (PE) are being investigated for their potential therapeutic properties.
Purpose of the Study:
- To investigate the mechanism of antiviral action of polystyrolsulfonate (PSS) and polyallylamine (PAA) against measles virus.
- To determine the effect of PSS with varying polymerization degrees and PAA on measles virus infectivity.
- To elucidate the interaction between PEs and measles virus surface proteins.
Main Methods:
- Measles virus (Leningrad-16 strain) was used.
- Virus infectious titers were determined after interaction with PSS (various polymerization degrees) and PAA using titration and cytopathogenic effect detection.
- Circular dichroism and fluorescence spectroscopy were employed to analyze viral protein structure changes.
Main Results:
- A significant reduction in measles virus infectious titers was observed with PSS (degree of polymerization 8) and PAA.
- Analysis revealed that PEs damage the secondary structure of viral proteins.
- PSS 8 interacts with viral proteins, disrupting alpha-helices and forming loops and tails.
Conclusions:
- The studied polyelectrolytes demonstrate potential as antiviral preparations against measles virus.
- Circular dichroism and protein fluorescence are effective methods for detecting viral protein structural damage by external agents.
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