Single-shot, simultaneous incoherent and holographic microscopy.

Area of Science:

• Optics and Photonics
• Microscopy
• Digital Holography

Background:

• Simultaneous acquisition of multiple image modalities is challenging.
• Digital holographic microscopy offers high-resolution imaging but often requires separate recordings for different signal types.
• Integrating incoherent and holographic imaging enhances microscopy capabilities.

Purpose of the Study:

• To demonstrate a single-shot method for simultaneous recording of incoherent and holographic (intensity and phase) images.
• To enable demultiplexing of these signals within the same camera frame.
• To showcase applications in combined microscopy techniques.

Main Methods:

• Utilizing carrier frequency modulation for signal separation in the spatial frequency domain.
• Leveraging spatial oversampling inherent in off-axis digital holographic configurations.
• Implementing a single-camera acquisition system.

Main Results:

• Successful single-shot recording of one incoherent and two holographic images.
• Effective demultiplexing of signals using the proposed spatial frequency domain approach.
• Demonstrated applicability to combined fluorescence and digital holographic microscopy.
• Demonstrated applicability to combined bright field and holographic second harmonic generation microscopy.

Conclusions:

• The developed technique allows for efficient, simultaneous capture of diverse image types.
• This method significantly advances combined imaging modalities in microscopy.
• The approach is versatile and applicable to various advanced microscopy techniques.