Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Composite Liquid Marble Templated Millimetric Capsule With Tunable Rigidity, Porosity, and Thermal Reconfigurability Toward 3D Cell Culture.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Engineered GelMA Microgels for Locoregional Doxorubicin Release and Apoptosis Induction in Oral Squamous Cell Carcinoma.

ACS biomaterials science & engineering·2026
Same author

Bioengineered In Vitro 3D Cancer Models: A New Paradigm in Ethical and Predictive Oncology Research Toward Successful Pre-Clinical Drug Screening.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Osteosarcoma-on-a-chip model mimicking intra-tumoral heterogeneity to interrogate tumor-associated macrophage reprogramming for immunotherapeutics.

Biomaterials·2025
Same author

Instant Protection Nanospray Bandage Driven Anti-Scar Wound Healing.

ACS applied materials & interfaces·2025
Same author

Correction: Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation.

PloS one·2025
Same journal

A Novel 3D Bioprinting Strategy for Bioengineering of Urethra with Clinical Relevance.

Tissue engineering. Part A·2026
Same journal

Hydrogel-Encapsulated Primed MSCs Enhance Regeneration in Full-Thickness Porcine Burn Wounds.

Tissue engineering. Part A·2026
Same journal

Unidirectional Porous Carbonate Apatite Fabricated by Gelatin-Based Freeze Casting for Bone Regeneration.

Tissue engineering. Part A·2026
Same journal

Regenerative Nanoscaffolds for Chronic Tympanic Membrane Perforation: From Bench to Clinical Translation.

Tissue engineering. Part A·2026
Same journal

Impact of IFN-γ-Pretreated Umbilical Cord Mesenchymal Stem Cells Implanted in Mesh on Pelvic Organ Prolapse.

Tissue engineering. Part A·2026
Same journal

The Driving Force of Hierarchical Collagen Fiber Formation: A Review of Tendon, Ligament, and Meniscus Mechanobiology.

Tissue engineering. Part A·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model
07:06

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model

Published on: October 10, 2025

Intervertebral disk tissue engineering using biphasic silk composite scaffolds.

Sang-Hyug Park1, Eun Seok Gil, Hongsik Cho

  • 1Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.

Tissue Engineering. Part A
|September 17, 2011
PubMed
Summary
This summary is machine-generated.

A novel biphasic scaffold using silk for annulus fibrosus and fibrin/hyaluronic acid for nucleus pulposus successfully regenerated intervertebral disc (IVD) tissue in vitro.

More Related Videos

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
07:28

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant

Published on: September 7, 2016

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

Related Experiment Videos

Last Updated: May 29, 2026

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model
07:06

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model

Published on: October 10, 2025

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
07:28

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant

Published on: September 7, 2016

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Intervertebral disc (IVD) regeneration requires restoring both annulus fibrosus (AF) and nucleus pulposus (NP) functions.
  • Existing scaffolds often lack native AF lamellar features or only address NP tissue.
  • A combined approach is crucial for complete IVD regeneration.

Purpose of the Study:

  • To develop and evaluate a biphasic biomaterial scaffold for in vitro IVD regeneration.
  • To mimic the distinct structural and cellular requirements of the AF and NP tissues.
  • To assess the efficacy of a silk-based AF component and a fibrin/hyaluronic acid (HA) NP component.

Main Methods:

  • Fabrication of toroidal silk scaffolds with lamellar and porous morphologies for AF.
  • Encapsulation of porcine AF cells in lamellar/porous silk scaffolds.
  • Development of fibrin/HA hydrogels for NP tissue engineering.
  • Co-culture of AF and NP components for 6 weeks to form a complete IVD construct.

Main Results:

  • Lamellar silk scaffolds supported AF-like tissue formation within 2 weeks.
  • Porcine chondrocytes adopted an NP phenotype within fibrin/HA hydrogels after 4 weeks.
  • The biphasic scaffold successfully promoted the formation of integrated AF and NP tissues in vitro.

Conclusions:

  • A biphasic scaffold combining silk-based AF and fibrin/HA-based NP is effective for IVD regeneration.
  • The lamellar silk structure is crucial for supporting AF tissue development.
  • This in vitro model demonstrates potential for future IVD repair strategies.