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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
Glycosilated nucleolin as marker for human gliomas
R Galzio1, F Rosati, E Benedetti
1Department of Health Sciences, University of L'Aquila, L'Aquila, Italy.
Journal of Cellular Biochemistry
|September 23, 2011
Summary
Surface nucleolin, a protein found on cell surfaces, increases with glioma malignancy. This finding suggests surface nucleolin could serve as a biomarker for grading gliomas and a target for cancer therapy.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Nucleolin is a key protein regulating gene expression and cell proliferation.
- While typically nucleolar, nucleolin can appear on the cell surface, particularly in cancer.
- Surface nucleolin has emerged as a potential target for anti-cancer therapies.
Purpose of the Study:
- To investigate the presence and localization of nucleolin in human gliomas.
- To correlate glycosylated surface nucleolin levels with glioma malignancy grade.
- To evaluate surface nucleolin as a potential diagnostic and therapeutic target in gliomas.
Main Methods:
- Analysis of glioma specimens and primary cell cultures at various malignancy grades.
- Utilized a custom-produced antibody against gp273 protein to detect glycosylated surface nucleolin.
- Correlated surface nucleolin expression with glioma histopathological grading.
Main Results:
- Surface nucleolin expression was found to increase with increasing glioma malignancy grade.
- Glycosylated surface nucleolin was detected on glioma cells.
- The study provides evidence for nucleolin's role in glioma progression.
Conclusions:
- Surface nucleolin expression correlates with glioma malignancy.
- Glycosylated surface nucleolin may serve as a valuable histopathological marker for glioma grading.
- Surface nucleolin presents a promising target for novel glioma-targeted therapies.
