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In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
Published on: June 10, 2016
Pancreatic islet and progenitor cell surface markers with cell sorting potential.
1Department of Beta-Cell Regeneration, Hagedorn Research Institute, Niels Steensens Vej 1, 2820 Gentofte, Denmark.
Diabetologia
|September 28, 2011
Summary
Researchers identified novel surface markers like DISP2 and DNER for pancreatic beta cells. Antibodies targeting these markers enable cell imaging and purification, crucial for stem cell-derived therapies.
Area of Science:
- Cell Biology
- Stem Cell Research
- Biomarker Discovery
Background:
- Pancreatic beta cells are vital for glucose regulation.
- Therapeutic applications require pure, functional beta cells.
- Identifying specific cell surface markers is key for cell isolation and differentiation.
Purpose of the Study:
- To identify novel surface biomarkers for pancreatic beta cells and their progenitors.
- To develop antibody tools for imaging and immunoisolation of these cells.
- To advance stem cell differentiation for therapeutic beta cell generation.
Main Methods:
- Bioinformatics-driven data mining to identify candidate genes.
- In situ hybridization and immunohistochemistry for validation.
- Antibody development and application in Fluorescence-Activated Cell Sorting (FACS).
Main Results:
- Identified SEZ6L2, LRP11, DISP2, and SLC30A8 in developing islet cells.
- Identified DDR1 and DNER in early pancreatic progenitors.
- Validated DISP2 and DNER antibodies for FACS-mediated cell purification.
Conclusions:
- Extracellular epitopes of SEZ6L2, LRP11, DISP2, DDR1, and DNER serve as valuable tags for pancreatic cell visualization.
- Antibodies against DISP2 and DNER are effective for FACS purification of pancreatic cells.
- These findings support the generation of therapeutic-grade human beta cells.
