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Related Experiment Video

Updated: May 29, 2026

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
05:21

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection

Published on: August 14, 2019

Imaging luciferase-expressing viruses.

Michael A Barry1, Shannon May, Eric A Weaver

  • 1Department of Internal Medicine, Mayo Clinic, Rochester, MN, USA. mab@mayo.edu

Methods in Molecular Biology (Clifton, N.J.)
|September 28, 2011
PubMed
Summary
This summary is machine-generated.

Luciferase imaging offers a powerful, noninvasive method for tracking cells and viruses in vivo. This technique aids in studying viral infections and monitoring tumor cell activity in preclinical models.

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Area of Science:

  • Biomedical Imaging
  • Molecular Biology
  • Preclinical Research

Background:

  • Optical imaging of luciferase gene expression is a key tool for in vivo studies.
  • It enables tracking of cells and viruses in small animal models.
  • Applications include studying viral infections and oncolytic therapies.

Purpose of the Study:

  • To describe techniques for luciferase imaging.
  • To apply these methods to track adenoviruses in vivo.
  • To discuss challenges in obtaining accurate imaging data.

Main Methods:

  • Utilizing luciferase imaging for in vivo tracking.
  • Applying methods to mouse and hamster models.
  • Focusing on replication-defective and replication-competent adenoviruses.

Main Results:

  • Demonstrated the utility of luciferase imaging for tracking viral distribution.
  • Showcased its application in oncolysis studies for monitoring tumor cell dynamics.
  • Highlighted the adaptability of the technique for various viral vectors.

Conclusions:

  • Luciferase imaging is a versatile tool for in vivo biological research.
  • Accurate data acquisition requires careful consideration of methodological caveats.
  • The described techniques support advancements in virology and oncology research.