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Classic in vivo cancer models: three examples of mouse models used in experimental therapeutics

Anna Kruczynski1, Bridget T Hill

  • 1Centre de Recherche Pierre Fabre, Cedex, France.

Current Protocols in Pharmacology
|October 1, 2011
PubMed

Insights

Transplantable animal tumors, including murine leukemia and melanoma, are crucial for evaluating new anticancer drugs. This study details protocols for using these models, alongside human tumor xenografts, in preclinical cancer research.

Area of Science:

  • Oncology
  • Pharmacology
  • Preclinical Cancer Research

Background:

  • Transplantable animal tumor models have historically driven the discovery of anticancer drugs.
  • These models remain vital for the rigorous evaluation of novel therapeutic agents.
  • Standardized protocols are essential for reproducible preclinical drug testing.

Purpose of the Study:

  • To describe established protocols for utilizing transplantable experimental tumors in drug evaluation.
  • To present methodologies for in vivo assessment of anticancer agent efficacy.
  • To highlight the utility of murine and human xenograft models in oncology research.

Main Methods:

  • Detailed protocols for intravenously-implanted murine P388 leukemia.
  • Methodologies for subcutaneously-implanted murine B16 melanoma.
  • Procedures for subcutaneously-implanted human tumor xenografts (LX-1 lung, MX-1 breast).

Main Results:

  • Established protocols enable consistent use of P388 leukemia, B16 melanoma, LX-1, and MX-1 models.
  • These models facilitate detailed pharmacokinetic and pharmacodynamic studies of anticancer agents.
  • The described methods support comparative efficacy assessments of novel drug candidates.

Conclusions:

  • The described protocols provide a foundation for robust preclinical anticancer drug evaluation.
  • Transplantable tumor models are indispensable tools in the development pipeline for new cancer therapies.
  • Utilizing both murine and human xenograft models offers comprehensive preclinical insights.

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