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Femoral Bone Marrow Aspiration in Live Mice
Published on: July 5, 2014
Perfusion method for harvesting bone marrow cells from dogs.
Masahiko Sato1, Yuko Goto-Koshino, Hiroyuki Mochizuki
1Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, Japan.
American Journal of Veterinary Research
|October 4, 2011
Summary
A new bone marrow perfusion method significantly reduces peripheral blood contamination and increases progenitor cell yield in dogs compared to aspiration. This makes perfusion a more suitable technique for harvesting canine bone marrow cells for transplantation.
Area of Science:
- Veterinary Medicine
- Cell Biology
- Hematology
Background:
- Conventional aspiration methods for harvesting canine bone marrow mononuclear cells (BMMCs) can lead to peripheral blood contamination.
- Efficient collection of progenitor cells is crucial for bone marrow transplantation.
Purpose of the Study:
- To compare the cellular composition and colony-forming capacity of BMMCs obtained from dogs using a novel perfusion technique versus the standard aspiration method.
- To evaluate the suitability of the perfusion method for clinical applications like bone marrow transplantation.
Main Methods:
- Bone marrow was harvested from 7 healthy adult Beagles using both perfusion and aspiration techniques.
- Flow cytometry was used to quantify peripheral blood cell contamination and CD34+ progenitor cell percentages.
- A colony-forming unit (CFU) assay assessed the number of progenitor cells.
Main Results:
- The perfusion method was safely implemented in all dogs.
- Perfusion significantly reduced the percentage of contaminating lymphocytes (CD3+CD4+, CD3+CD8+, CD21+) compared to aspiration.
- Significantly higher percentages of CD34+ progenitor cells and CFU counts were achieved with the perfusion method.
Conclusions:
- The perfusion method effectively minimizes peripheral blood contamination in canine BMMC samples.
- Perfusion is a more efficient method for collecting canine bone marrow progenitor cells than aspiration.
- The perfusion technique is a promising alternative for harvesting bone marrow for transplantation in dogs.
