Rapid vibrational imaging with sum frequency generation microscopy
Related Experiment Video
Updated: May 28, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
Published on: December 1, 2023
Varun Raghunathan1, Yang Han, Olaf Korth
1Department of Chemistry, University of California at Irvine, Irvine, California 92627-2025, USA.
Abstract:
We demonstrate rapid vibrational imaging based on sum frequency generation (SFG) microscopy with a collinear excitation geometry. Using the tunable picosecond pulses from a high-repetition-rate optical parametric oscillator, vibrationally selective imaging of collagen fibers is achieved with submicrometer lateral resolution. We furthermore show simultaneous SFG and second harmonic generation imaging to emphasize the compatibility of the microscope with other nonlinear optical modalities.
Related Concept Videos
Super-resolution Fluorescence Microscopy
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Total Internal Reflection Fluorescence Microscopy
Confocal Fluorescence Microscopy

