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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 28, 2026

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Glycan microarrays.

Xuezheng Song1, Jamie Heimburg-Molinaro, David F Smith

  • 1Department of Biochemistry and The Glycomics Center, Emory University School of Medicine, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 4, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a method for creating fluorescent glycan microarrays. These microarrays enable efficient screening of glycan-binding proteins (GBPs) like lectins and antibodies.

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Last Updated: May 28, 2026

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Area of Science:

  • Carbohydrate Chemistry
  • Biotechnology
  • Immunology

Background:

  • Glycan microarrays are crucial tools for analyzing interactions between carbohydrates and proteins.
  • Current methods for creating glycan microarrays require specific derivatization techniques.
  • Standardization of glycan printing concentrations is essential for reliable data.

Purpose of the Study:

  • To describe a novel method for fluorescent and functional derivatization of free reducing glycans.
  • To detail the process of printing these derivatized glycans onto slides to create microarrays.
  • To demonstrate the interrogation of these newly developed glycan microarrays using glycan-binding proteins (GBPs).

Main Methods:

  • Fluorescent and functional derivatization of free reducing glycans.
  • Printing of derivatized glycans onto activated glass slides at consistent concentrations.
  • Screening of the generated glycan microarrays with various GBPs, including lectins, antibodies, bacteria, and viruses.

Main Results:

  • Successful creation of fluorescent glycan microarrays with uniformly printed glycans.
  • Demonstration of the utility of these microarrays in identifying GBP binding specificities.
  • The method allows for the characterization of interactions between a wide range of GBPs and glycans.

Conclusions:

  • The described method provides a robust platform for generating fluorescent glycan microarrays.
  • This technique facilitates high-throughput screening and characterization of glycan-GBP interactions.
  • The developed glycan microarrays are valuable tools for glycobiology research and diagnostics.