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Updated: May 28, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
[Sandfly virus meningitis in a Danish traveller returning from Tuscany]
Nanna Bang Nissen1, Sanne Jespersen, Lasse Vinner
1Infektionsmedicinsk Afdeling, Aarhus Universitetshospital, Skejby, 8200 Aarhus N, Denmark. nannanissen@hotmail.com
Abstract:
We report the first case of Sandfly virus meningitis in a Danish traveller returning from Tuscany. A 52 year-old man was admitted with headache, fever and photophobia. Spinal fluid showed evidence of aseptic meningitis. Indirect immuno-fluorescence assays showed presence of immunoglobulin (Ig)M and IgG antibodies reactive against Toscana virus, and Phlebovirus RNA was detected in blood by real-time polymerase chain reaction. The patient recovered spontaneously. Since Sandfly virus is a very common cause of meningitis in the Mediterranean countries, it is important to be aware of this disease in travellers returning from these areas.
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