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Updated: May 28, 2026
08:59
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
Published on: September 27, 2019
Functionalization of DNA nanostructures with proteins.
Barbara Saccà1, Christof M Niemeyer
1Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Dortmund, Germany.
Chemical Society Reviews
|October 7, 2011
Summary
Researchers created DNA-protein conjugates by chemically linking proteins with synthetic DNA. These novel biomolecular tools offer enhanced functionality for applications in diagnostics and DNA-based nanotechnology.
Area of Science:
- Biomolecular Nanosciences
- Chemical Biology
- Synthetic Biology
Background:
- Proteins possess evolved, intrinsic functionalities.
- Natural protein functions can be augmented using synthetic modifications.
- Artificial nucleic acids offer robust and specific tagging capabilities.
Purpose of the Study:
- To review chemical strategies for synthesizing DNA-protein conjugates.
- To explore the applications of these conjugates in biomolecular nanosciences.
- To highlight the advantages of DNA-protein conjugation for advanced applications.
Main Methods:
- Chemical conjugation of proteins with synthetic nucleic acids.
- Utilizing Watson-Crick hybridization for specific addressing of the DNA tag.
- Review of existing literature on synthesis and applications (96 references).
Main Results:
- Successful creation of DNA-protein conjugates with enhanced functionalities.
- Demonstrated versatility of these conjugates in diverse applications.
- Established the synthetic accessibility and robustness of the DNA tag.
Conclusions:
- DNA-protein conjugates represent a powerful platform for functionalizing proteins.
- These conjugates are valuable tools for biomedical diagnostics and DNA-based nanofabrication.
- Chemical synthesis approaches enable the creation of these advanced biomolecular constructs.
Related Concept Videos
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
00:58DNA Packaging
Overview