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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

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Super-atmospheric pressure electrospray ion source: applied to aqueous solution.

Lee Chuin Chen¹, Mridul Kanti Mandal, Kenzo Hiraoka

¹Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan. leechuin.yamanashi@gmail.com

Journal of the American Society for Mass Spectrometry

|October 13, 2011

Summary

This study presents a novel pressurized electrospray ionization (ESI) ion source directly coupled to a mass spectrometer. This improved design enhances ion transmission and enables sensitive detection of native proteins.

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

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Published on: July 12, 2013

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Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Area of Science:

Analytical Chemistry
Mass Spectrometry

Background:

Previous electrospray ionization (ESI) ion source operated at pressures above atmospheric pressure.
Direct coupling to mass spectrometer was lacking, leading to significant ion loss.

Purpose of the Study:

To report a new prototype design of a pressurized ESI ion source.
To directly couple the ion source to a mass spectrometer using a modified ion transport capillary.
To demonstrate sensitive detection of native proteins.

Main Methods:

Development of a second-generation pressurized ESI ion source.
Integration of a modified ion transport capillary for direct coupling to the mass spectrometer's first pumping stage.
Operation at elevated pressures to enhance stability and ion generation.

Main Results:

Successful direct coupling of the pressurized ESI ion source to the mass spectrometer without system modification.
Stable electrospray achieved for high surface tension solutions without electric discharge.
Sensitive detection of native proteins in both positive and negative ion modes demonstrated.

Conclusions:

The new design significantly improves ion transmission efficiency.
Direct coupling enhances the sensitivity of protein detection using pressurized ESI.
The system is effective for analyzing native proteins in aqueous solutions.