Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards point-of-care tests for protein detection at the attomolar level via disposable pollen-based nanoplasmonic probes grafted with polymer-based receptors.

Biomedical optics express·2026
Same author

Development of a portable device for the detection of anti-PEG antibodies in human plasma.

Scientific reports·2026
Same author

Correction: Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis.

Molecular neurodegeneration·2026
Same author

Biocompatible Self-Healing Hydrogel for VAT 3D Printing.

ACS materials Au·2026
Same author

Assessing Natural Fillers as Substitutes for Glass Fibers in Polyamide 6 Composites for Large-Format Additive Manufacturing.

Polymers·2026
Same author

Towards point-of-care tests: Comparative study of different antibody densities for plasmonic surfaces to achieve custom IL-18 biosensors.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: May 28, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Solid phase DNA extraction on PDMS and direct amplification.

Laura Pasquardini1, Cristina Potrich, Marzia Quaglio

  • 1FBK-Fondazione Bruno Kessler, Center for Materials and Microsystems, Povo, Trento, Italy. pasqua@fbk.eu

Lab on a Chip
|October 13, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel Poly(DiMethyl)Siloxane microfluidic device for simultaneous DNA purification from blood and Polymerase Chain Reaction (PCR) amplification, eliminating the need for elution and passivation agents.

More Related Videos

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic &#8216;Touch DNA&#8217; Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

Related Experiment Videos

Last Updated: May 28, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic &#8216;Touch DNA&#8217; Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

Area of Science:

  • Biotechnology
  • Materials Science
  • Molecular Biology

Background:

  • Microfluidic devices offer miniaturized platforms for biological sample processing.
  • Traditional DNA purification and amplification protocols involve multiple steps, including elution and surface treatments.
  • Poly(DiMethyl)Siloxane (PDMS) is a versatile polymer commonly used in microfluidics.

Purpose of the Study:

  • To develop an integrated microfluidic device for simultaneous DNA purification and PCR amplification.
  • To leverage the intrinsic properties of PDMS for simplified DNA preparation.
  • To eliminate the elution step in DNA purification and surface passivation in PCR.

Main Methods:

  • Fabrication of a novel microfluidic device using Poly(DiMethyl)Siloxane (PDMS).
  • Characterization of PDMS surface chemistry using X-Ray Photoelectron Spectroscopy (XPS).
  • Assessment of PDMS nanomorphology using Atomic Force Microscopy (AFM) and Field Emission Scanning Electron Microscopy (FESEM).
  • Confocal fluorescence microscopy to analyze DNA distribution.

Main Results:

  • Demonstrated spontaneous DNA adsorption onto the PDMS surface due to its nanomorphology.
  • Achieved direct DNA purification from blood and subsequent PCR amplification within a single reaction chamber.
  • Eliminated the requirement for DNA elution and surface passivation agents for PCR.

Conclusions:

  • The developed PDMS microfluidic device enables a simplified, integrated workflow for DNA preparation from complex biological samples.
  • The intrinsic material properties of PDMS can be exploited to overcome common challenges in microfluidic DNA analysis.
  • This innovation significantly streamlines sample-to-answer processes in molecular diagnostics.