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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: May 28, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Development and utilization of non-coding RNA-small molecule interactions.

Wesleigh E Georgianna1, Douglas D Young

  • 1Duke Translational Research Institute, Duke University, Durham, NC 27715, USA.

Organic & Biomolecular Chemistry
|October 14, 2011
PubMed
Summary
This summary is machine-generated.

Small molecules can regulate non-coding RNAs (ncRNAs), which are vital for gene regulation and implicated in diseases. This research explores small molecule regulators for ncRNAs like miRNA and siRNA, advancing molecular therapeutics.

More Related Videos

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

Related Experiment Videos

Last Updated: May 28, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Non-coding RNAs (ncRNAs) are critical for gene regulation beyond protein synthesis.
  • Aberrant ncRNA activity is linked to various disease states.
  • ncRNAs present a promising target for therapeutic intervention.

Purpose of the Study:

  • To provide an overview of small molecule regulators targeting specific ncRNAs.
  • To highlight recent advancements in the exogenous regulation of ncRNAs by small molecules.
  • To discuss the therapeutic potential of small molecule-ncRNA interactions.

Main Methods:

  • Literature review of recent findings on small molecule regulation of ncRNAs.
  • Focus on specific ncRNA classes including miRNA, siRNA, RNA aptamers, riboswitches, and ribozymes.
  • Analysis of exogenous small molecule interactions with ncRNA targets.

Main Results:

  • Significant progress has been made in identifying and developing small molecule regulators for ncRNAs.
  • Exogenous small molecules demonstrate efficacy in modulating the function of various ncRNAs.
  • The study details regulatory mechanisms for miRNA, siRNA, RNA aptamers, riboswitches, and ribozymes.

Conclusions:

  • Small molecules offer a powerful approach for regulating ncRNA function.
  • The development of these molecular tools has significant implications for advancing molecular therapeutics.
  • Targeting ncRNAs with small molecules opens new avenues for disease treatment.