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Identification of Yersinia species by the API 20E
N K Sharma1, P W Doyle, S A Gerbasi
1McNair Clinical Laboratories, Vancouver, British Columbia, Canada.
Journal of Clinical Microbiology
|June 1, 1990
Summary
The API 20E system accurately identified 90% of Yersinia isolates, showing high effectiveness for Yersinia enterocolitica and Yersinia frederiksenii. However, Yersinia intermedia identification results were found to be unacceptable.
Area of Science:
- Microbiology
- Clinical Diagnostics
Background:
- Accurate identification of Yersinia species is crucial for clinical and epidemiological purposes.
- The API 20E system is a widely used biochemical method for bacterial identification.
Purpose of the Study:
- To evaluate the performance of the API 20E system in identifying Yersinia isolates.
- To determine the accuracy and reliability of the API 20E for key Yersinia species.
Main Methods:
- A prospective study involving 183 Yersinia isolates.
- Incubation of isolates at 28°C for 18 to 24 hours.
- Identification using the API 20E system.
Main Results:
- An overall correct identification rate of 90% was achieved.
- Positive predictive values were 94% for Yersinia enterocolitica and 92% for Yersinia frederiksenii.
- Identification of Yersinia intermedia yielded unacceptable results.
Conclusions:
- The API 20E system demonstrates good performance for identifying common Yersinia species like Y. enterocolitica and Y. frederiksenii.
- The system's reliability is compromised for Yersinia intermedia identification.
- Further investigation or alternative methods are needed for accurate Y. intermedia detection.