Highly sensitive and selective uric acid biosensor based on RF sputtered NiO thin film.

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Uric acid detection is crucial for diagnosing conditions like gout and Lesch-Nyhan syndrome.
• Developing sensitive and selective biosensors is essential for accurate uric acid monitoring.
• Nickel oxide (NiO) thin films offer promising properties for biosensor applications.

Purpose of the Study:

• To investigate the potential of RF sputtered NiO thin film as a matrix for a uric acid biosensor.
• To immobilize uricase enzyme onto the NiO matrix for enhanced biosensing capabilities.
• To evaluate the sensing performance of the developed uric acid biosensor.

Main Methods:

• Fabrication of NiO thin films on platinum-coated glass substrates.
• Immobilization of uricase enzyme onto the NiO surface via physical adsorption.
• Electrochemical analysis using cyclic voltammetry.
• Spectroscopic analysis using UV-visible spectroscopy.

Main Results:

• The bioelectrode (uricase/NiO/Pt/Ti/glass) exhibited high sensitivity (1278.48 μA/mM) and good linearity (0.05-1.0 mM).
• A low Michaelis-Menten constant (0.17 mM) indicated high affinity of uricase for uric acid.
• The NiO matrix provided excellent electron transport and nanoporous morphology for effective enzyme loading.

Conclusions:

• RF sputtered NiO thin films are suitable matrices for highly sensitive and selective uric acid biosensors.
• The developed bioelectrode demonstrates efficient uric acid sensing capabilities.
• The NiO matrix enhances enzyme loading and electron transport, leading to improved biosensor performance.