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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Pyrosequencing analysis of the apical root canal microbiota
José F Siqueira1, Flávio R F Alves, Isabela N Rôças
1Department of Endodontics and Molecular Microbiology Laboratory, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil. jf_siqueira@yahoo.com
Journal of Endodontics
|October 18, 2011
Summary
This study reveals a high diversity of bacterial species within the apical portion of infected root canals, crucial for understanding apical periodontitis. The research utilized massive parallel pyrosequencing to analyze bacterial communities.
Area of Science:
- Microbiology
- Endodontics
- Oral Health
Background:
- Bacterial biofilms in root canals are key to apical periodontitis.
- Understanding apical endodontic microbiota is crucial for pathogenesis.
Purpose of the Study:
- To investigate the diversity of the apical endodontic microbiota.
- To analyze bacterial communities in infected root canals using pyrosequencing.
Main Methods:
- Ten extracted teeth with apical periodontitis lesions were used.
- Apical root fragments were cryogenically ground for DNA extraction.
- Massive parallel pyrosequencing (16S rRNA gene) was performed.
Main Results:
- 187 bacterial species-level phylotypes, 84 genera, and 10 phyla were identified.
- Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria were the most prevalent phyla.
- High interindividual variation in apical microbiota composition was observed.
Conclusions:
- This study provides an extensive description of bacterial diversity in the apical root canal.
- A previously unrecognized high bacterial diversity in apical periodontitis was revealed.
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