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Towards multiple readout application of plasmonic arrays.

Dana Cialla¹, Karina Weber¹, René Böhme¹

¹Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany.

Beilstein Journal of Nanotechnology

|October 18, 2011

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Researchers developed a novel nanostructured gold surface for combined fluorescence and surface-enhanced Raman spectroscopy (SERS) detection. This platform enables sensitive detection of fluorescent molecules and their specific Raman fingerprints on a single chip.

Area of Science:

Nanotechnology
Spectroscopy
Bioanalytical Chemistry

Background:

Combining fluorescence and surface-enhanced Raman spectroscopy (SERS) offers complementary detection advantages.
Existing platforms face challenges in optimizing both fluorescence and SERS signals simultaneously due to distance-dependent quenching and enhancement effects.

Purpose of the Study:

To establish a nanostructured gold surface platform for the simultaneous detection of fluorescence and SERS signals.
To enable the binding of molecules at varying distances from the metallic surface to balance SERS enhancement and fluorescence quenching.

Main Methods:

Fabrication of plasmonic arrays with tailored nanostructures on a gold surface.
Utilizing gold nanostructured arrays to control molecular binding distances.

Keywords:

fluorescence multiple readout plasmonic array surface-enhanced Raman spectroscopy (SERS)surface-enhanced fluorescence (SEF)

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Demonstrating simultaneous SERS and fluorescence detection via a DNA detection scheme.

Main Results:

A nanostructured gold surface was successfully fabricated, allowing for dual fluorescence and SERS detection.
The design permits molecules to bind at different distances, optimizing the trade-off between SERS enhancement and fluorescence quenching.
Successful demonstration of simultaneous SERS and fluorescence detection using gold nanostructured arrays for DNA detection.

Conclusions:

The developed nanostructured gold surface platform effectively combines fluorescence and SERS detection on a single chip.
This approach allows for sensitive detection of both fluorescence and specific Raman fingerprints of molecules.
The findings pave the way for more versatile applications of plasmonic arrays in bioanalysis.