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Updated: May 28, 2026
15:41
Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
Published on: October 12, 2009
Phosphoproteomic profiling of the myocyte
Alistair V G Edwards1, Stuart J Cordwell, Melanie Y White
1Discipline of Pathology, School of Medical Sciences, The University of Sydney, New South Wales, Australia.
Circulation. Cardiovascular Genetics
|October 20, 2011
Summary
This study highlights the critical role of protein phosphorylation in myocytes and introduces advanced phosphoproteomics techniques for comprehensive myocardial analysis. These methods offer deeper insights into cellular processes and cardiovascular disease research.
Area of Science:
- Cardiovascular Research
- Cellular Biology
- Proteomics
Background:
- Protein phosphorylation is vital for myocyte functions like metabolism and signaling.
- Traditional targeted studies offer a limited view of cellular phosphorylation.
- Recent advances enable comprehensive analysis of the phosphoproteome.
Purpose of the Study:
- To address the lack of global, high-throughput myocardial phosphoproteome characterization.
- To present high-resolution strategies for identifying, localizing, and quantifying thousands of phosphorylation sites.
- To discuss current phosphoproteomic investigations in myocytes.
Main Methods:
- Utilizing affinity enrichment followed by liquid chromatography and mass spectrometry.
- Moving beyond gel-based technologies for phosphoproteomics.
- Describing current best methods and their applications in myocyte subcompartments.
Main Results:
- Phosphoproteomics provides a deeper view of cellular phosphorylation events.
- Advanced techniques enable high-throughput characterization of the myocardial phosphoproteome.
- Current investigations focus on myocytes and their subcompartments.
Conclusions:
- Comprehensive myocardial phosphoproteome cataloging is achievable with specialized techniques.
- Phosphoproteomics offers significant biological insight for cardiovascular research.
- This approach is crucial for understanding physiological and diseased states in myocytes.
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