Related Experiment Video
Updated: May 28, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
Published on: April 6, 2016
Exploration of a new concept for automated dried blood spot analysis using flow-through desorption and online
Jan Albert Ooms1, Lena Knegt, Emile Hermannus Maarten Koster
1Spark Holland B.V., Pieter de Keyserstraat 8, Emmen, The Netherlands. bert.ooms@sparkholland.com
Background:
A new concept for simple and generic automation of dried blood spot (DBS) analysis is evaluated. Flow-through desorption of the blood spot is coupled online to SPE and MS/MS without using a LC column. Blood, spiked with a mixture of test compounds is spotted on paper, dried and then desorbed by means of a prototype clamping device.
Results:
Conditions for extraction and chromatography on a single SPE cartridge were optimized with respect to clean-up, separation and ionization suppression. Addition of internal standard using loop-injection upstream of the clamped blood spot was examined and proved to be simple and reliable. Validation results for the 1-1000 ng/ml range are well within acceptance criteria for bioanalysis.
Conclusion:
Results demonstrate feasibility of the DBS SPE-MS/MS concept for efficient automation of the entire DBS analysis workflow.
More Related Videos
11:28Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Published on: March 13, 2015
07:55Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
Published on: June 21, 2019
Related Concept Videos
MALDI-TOF Mass Spectrometry
Tandem Mass Spectrometry