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Updated: May 28, 2026

Assembly and Characterization of Polyelectrolyte Complex Micelles
Published on: March 2, 2020
Structural characterization of casein micelles: shape changes during film formation
R Gebhardt1, C Vendrely, U Kulozik
1Technische Universität München, Chair for Food Process Engineering and Dairy Technology, Weihenstephaner Berg 1, 85354 Freising-Weihenstephan, Germany. ronald.gebhardt@tum.de
Abstract:
The objective of this study was to determine the effect of size-fractionation by centrifugation on the film structure of casein micelles. Fractionated casein micelles in solution were asymmetrically distributed with a small distribution width as measured by dynamic light scattering. Films prepared from the size-fractionated samples showed a smooth surface in optical microscopy images and a homogeneous microstructure in atomic force micrographs. The nano- and microstructure of casein films was probed by micro-beam grazing incidence small angle x-ray scattering (μGISAXS). Compared to the solution measurements, the sizes determined in the film were larger and broadly distributed. The measured GISAXS patterns clearly deviate from those simulated for a sphere and suggest a deformation of the casein micelles in the film.
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