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Updated: May 28, 2026
Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
Published on: March 11, 2020
[Construction of expression vector driven by tobacco root-specific promoter and transformation into Rhodiola hairy
Yao-hui Hu1, Bei-bei Liu, Han-song Yu
1College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China. huyaohui@vip.163.com
Objective:
To construct root-specific promoter of plant expression vector and transformate into Rhodiola hairy root.
Methods:
The expression vector pCA-Tob7: UGTR, driven by tobacco root-specific promoter TobRB7, was constructed from pCAM-BIA1301 by substituting the CaMV 35S promoter and GUS gene with TobRB7 and UGTR(a glycosyltransferase gene), respectively. The pCA-Tob7: UGTR vector was introduced into Agrobacterium tumefaciens strain, and the hairy root of Rhodiola were then transformed.
Results:
Some Kanamycin resistant plants were positive,which indicated that the expression vector had integrated into Rhodiola hairy root genome.
Conclusion:
Root-specific promoter of plant expression vector is constructed and the hairy root of Rhodiola are transformed for future research.
