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Related Concept Videos

Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

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Related Experiment Video

Updated: May 28, 2026

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
10:02

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Published on: October 23, 2016

Clathrin-mediated endocytosis in budding yeast.

Jasper Weinberg1, David G Drubin

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA.

Trends in Cell Biology
|October 25, 2011
PubMed
Summary

Clathrin-mediated endocytosis in yeast involves ~60 proteins. Live-cell microscopy and yeast genetics reveal their ordered assembly, function, and disassembly, advancing our understanding of this vital cellular process.

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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

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Last Updated: May 28, 2026

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

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In vivo and in vitro Studies of Adaptor-clathrin Interaction
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In vivo and in vitro Studies of Adaptor-clathrin Interaction

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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Published on: December 6, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Clathrin-mediated endocytosis (CME) is a fundamental cellular process for internalizing molecules at the plasma membrane.
  • In budding yeast Saccharomyces cerevisiae, CME requires the coordinated action of approximately 60 proteins.
  • Understanding the dynamic regulation of these protein interactions is crucial for cell function.

Purpose of the Study:

  • To review recent advancements in understanding the molecular mechanisms of CME in Saccharomyces cerevisiae.
  • To highlight the methodologies used to analyze protein dynamics during yeast endocytosis.
  • To discuss the regulation and disassembly of the endocytic machinery.

Main Methods:

  • Two-color live-cell fluorescence microscopy for in vivo analysis of endocytic protein dynamics.
  • Yeast genetics and functional genomics to identify protein interaction networks and regulators.
  • Quantitative data analysis enabling theoretical modeling of endocytic pathways.

Main Results:

  • Live-cell microscopy effectively identifies new endocytic components and determines protein arrival/dissociation order.
  • Yeast genetics reveals complex interaction networks governing endocytic protein function.
  • Quantitative data supports the development of theoretical models for CME regulation.

Conclusions:

  • Recent findings have significantly advanced the understanding of CME protein recruitment, function, and disassembly in yeast.
  • The study emphasizes the power of combining advanced microscopy, genetics, and computational modeling.
  • Budding yeast serves as a powerful model system for dissecting complex cellular processes like endocytosis.