Related Experiment Video
Updated: May 28, 2026
06:25
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Recent applications in chiral high performance liquid chromatography: a review
Alberto Cavazzini1, Luisa Pasti, Alessandro Massi
1University of Ferrara, Department of Chemistry, 44121 Ferrara, Italy. cvz@unife.it
Analytica Chimica Acta
|October 26, 2011
Summary
This review covers key chiral stationary phases (CSPs) used in high-performance liquid chromatography (HPLC). It highlights recent advancements in enantiorecognition mechanisms, new materials, and applications for chiral separations.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Chiral stationary phases (CSPs) are crucial for enantioselective separations in high-performance liquid chromatography (HPLC).
- Understanding CSPs is vital for advancing chiral analysis and purification.
Purpose of the Study:
- To review the most important and widely used CSPs in HPLC.
- To summarize recent contributions in enantiorecognition mechanisms, new CSP materials, and novel applications.
- To identify emerging CSPs with potential for future chiral separation technologies.
Main Methods:
- Literature review of recent publications on CSPs for HPLC.
- Classification of reviewed works into enantiorecognition mechanisms, new materials, and new applications.
- Focus on emerging CSPs and their characteristics.
Main Results:
- Overview of established and broadly used CSPs in HPLC.
- Summary of recent research focusing on understanding enantiorecognition.
- Identification of novel CSP materials and their applications in chiral separations.
- Highlighting promising new CSPs for future chiral separation challenges.
Conclusions:
- Recent advancements have expanded the utility and understanding of CSPs in HPLC.
- Emerging CSPs show significant promise for future chiral separation applications.
- Continued research into CSPs is essential for progress in enantioselective chromatography.
Related Concept Videos
01:11High-Performance Liquid Chromatography: Introduction
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
00:57High-Performance Liquid Chromatography: Instrumentation
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
01:05High-Performance Liquid Chromatography: Elution Process
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
01:13Principles Of Column Chromatography
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
01:15High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
01:18Supercritical Fluid Chromatography
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
SFC utilizes a supercritical fluid mobile phase,...