In vivo two-photon microscopy to 1.6-mm depth in mouse cortex
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Demirhan Kobat1, Nicholas G Horton, Chris Xu
1Cornell University, School of Applied and Engineering Physics, Ithaca, New York 14853, USA. dk287@cornell.edu
Journal of Biomedical Optics
|October 28, 2011
Abstract:
Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.6 mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.
