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Related Experiment Videos

[Isolation of Staphylococcus aureus protoplasts using lysoamidase].

V V Petrov, E N Ratner, A I Severin

    Prikladnaia Biokhimiia I Mikrobiologiia
    |May 1, 1990
    PubMed
    Summary

    Lysoamidase effectively creates Staphylococcus aureus protoplasts, with optimal yield achieved during logarithmic growth. These protoplasts retain significant respiratory activity and can regenerate cell walls.

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    Area of Science:

    • Microbiology
    • Enzymology

    Context:

    • Staphylococcus aureus is a significant human pathogen.
    • Bacteriolytic enzymes offer potential therapeutic applications.

    Purpose:

    • To investigate the efficacy of lysoamidase on Staphylococcus aureus 209P.
    • To determine optimal conditions for protoplast formation and characterize their properties.

    Summary:

    • Lysoamidase treatment of Staphylococcus aureus 209P cells was studied using spectrophotometric, biochemical, and electron microscopic methods.
    • Optimal protoplast isolation occurred during the logarithmic growth phase, yielding 80% with 1 M sucrose stabilization.
    • Lysoamidase induced cell wall disruption, forming osmotically fragile spheroplasts and releasing viable protoplasts with 85-90% respiration activity and regenerative capacity.

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    Impact:

    • Establishes lysoamidase as a tool for S. aureus protoplast generation.
    • Provides insights into bacterial cell wall lysis and protoplast viability.
    • Informs potential applications of lysoamidase in microbiology and biotechnology.