Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A modified technique to process peripheral nerves for light microscopy.

T V Rao1, Y Ramamohan

  • 1Department of Neuropathology, National Institute of Mental Health & Neuro Sciences, Bangalore.

Indian Journal of Pathology & Microbiology
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraventricular neurocytoma : a clinical and electro-microscopic study.

Neurology India·2018
Same author

Is infantile spinal muscular atrophy a disease of maturation arrest or a dynamic neurogenic atrophy of the skeletal muscle?

Neurology India·2018
Same author

Genetic affinities between the migrant and parental populations of fishermen, East coast, India.

American journal of human biology : the official journal of the Human Biology Council·2017
Same author

Alzheimers disease - histological ultrastructural and immunochemical study of an autopsy proven case.

Indian journal of psychiatry·2011
Same author

The relevance of clinical autopsies in a psychiatric institute.

Indian journal of psychiatry·2011
Same author

Prevalence and distribution of cardiovascular risk factors in an urban industrial population in south India: a cross-sectional study.

The Journal of the Association of Physicians of India·2008
Same journal

Prof Aseem Kumar Basu, MBBS, DTM and H, FRCPath (Lond), PhD (Lond), Fmr. Professor Department of Haematology, School of Tropical Medicine, Kolkata, India.

Indian journal of pathology & microbiology·2026
Same journal

Beyond the ordinary: Extramedullary hematopoiesis in an ossified psammomatous meningioma.

Indian journal of pathology & microbiology·2026
Same journal

Histomorphological spectrum of Adnexal Tumor with rippled pattern an unusual variant: Report of three cases.

Indian journal of pathology & microbiology·2026
Same journal

The shape-shifter: A case of pleomorphic giant cell carcinoma of prostate.

Indian journal of pathology & microbiology·2026
Same journal

Metachronous renal cell carcinoma with delayed testicular metastasis coexisting with synchronous prostatic adenocarcinoma.

Indian journal of pathology & microbiology·2026
Same journal

Cribriform-morular thyroid carcinoma with nodal metastasis: A diagnostic dilemma.

Indian journal of pathology & microbiology·2026
See all related articles

This study introduces a novel method for processing peripheral nerve biopsies for light microscopy. The technique ensures uniformly thin sections, improving diagnostic accuracy and documentation for neuropathology.

Area of Science:

  • Neuropathology
  • Histotechnology
  • Microscopy techniques

Background:

  • Routine peripheral nerve biopsies require effective fixation, embedding, and sectioning for accurate light microscopy.
  • Current methods may present challenges in achieving uniformly thin sections suitable for detailed analysis.

Purpose of the Study:

  • To describe a new, streamlined technique for preparing peripheral nerve biopsies for light microscopy.
  • To enhance the quality of sections for improved diagnostic observation and documentation.

Main Methods:

  • Utilized buffered glutaraldehyde (pH 7.4) as a common fixative for both light and electron microscopy.
  • Employed a 'reverse embedding' method for paraffin embedding of nerve tissue.
  • Trimming blocks to cut uniformly thin sections on an ultramicrotome using glass knives.

Related Experiment Videos

Main Results:

  • The described technique yields uniformly thin sections of peripheral nerve biopsies.
  • These thin sections are compatible with various special stains.
  • The high resolution of the sections facilitates enhanced observation and documentation.

Conclusions:

  • The new technique offers an efficient and effective method for processing peripheral nerve biopsies for light microscopy.
  • Improved section quality leads to better diagnostic capabilities in neuropathology.
  • This method supports detailed histological analysis and accurate documentation of nerve pathology.