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Studies on steroid binding proteins in normal tissues and tumor cell lines
J Reventós1, G L Hammond, N A Musto
1Population Council Rockefeller University, New York, NY 10021.
Revista Espanola De Fisiologia
|March 1, 1990
Summary
Steroid binding proteins transport hormones. Recent advances in DNA technology allow detailed analysis of corticosteroid binding globulin, testosterone estradiol binding globulin, and androgen binding protein genes and their mRNA regulation.
Area of Science:
- Biochemistry
- Molecular Biology
- Endocrinology
Background:
- Steroid binding proteins transport steroid hormones extracellularly.
- Physicochemical properties and functions have been studied for over 50 years.
- Recombinant DNA technology has enabled new analyses of these proteins.
Purpose of the Study:
- To review recent findings on steroid binding proteins.
- To cover cloning and sequencing of cDNAs and genes for key binding proteins.
- To examine mRNA tissue distribution and regulation in normal and cancerous tissues.
Main Methods:
- Cloning and sequencing of cDNAs and genes.
- Analysis of messenger RNA (mRNA) tissue distribution.
- Investigation of mRNA regulation in various cell lines.
Main Results:
- cDNAs and genes for corticosteroid binding globulin, testosterone estradiol binding globulin, and androgen binding protein have been cloned and sequenced.
- Tissue distribution of mRNAs for these proteins has been determined.
- Regulation of mRNA synthesis in normal tissues and cancer cell lines has been analyzed.
Conclusions:
- Recent advances provide a deeper understanding of steroid binding protein gene structure and regulation.
- This knowledge is crucial for understanding steroid hormone transport and its role in health and disease.
- Further research can explore therapeutic strategies targeting these proteins.