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Published on: January 30, 2016
Calibration method of tilt and azimuth angles for alignment of TEM tomographic tilt series
Misa Hayashida1, Shinya Terauchi, Toshiyuki Fujimoto
1National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8565, Japan. misa-hayashida@aist.go.jp
Abstract:
This paper describes the calibration method of the tilt and azimuth angles of specimen using a digital protractor and a laser autocollimator for alignment of electron tomography. It also suggests an easy method to check whether the specimen is tilted by 180.0°, and whether the azimuth angle is 0.0°; the method involves the use of two images of a rod-shaped specimen collected before and after a 180.0° tilt. The method is based on the assumption that these images are symmetric about the tilt axis when the azimuth angle is 0.0°. In addition, we used an experiment to demonstrate the effect of the incorrect angles on reconstructed images and simulated the image quality against distance away from tilt axis.
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