Glycosylated self-assembled monolayers for arrays and surface analysis

Area of Science:

• Carbohydrate chemistry and glycobiology
• Surface science and nanotechnology
• Biomolecular interaction analysis

Background:

• Carbohydrates (glycans) play vital roles in biological processes, including disease and cell signaling.
• Glycomics research requires advanced tools like carbohydrate microarrays for studying glycan interactions.
• Effective surface modification is critical for high-performance glycan microarrays.

Purpose of the Study:

• To present versatile methods for preparing high-quality glycosylated self-assembled monolayers (glyco-SAMs).
• To enable rigorous characterization of glycan surface chemistries for glycomics.
• To facilitate the exploration of immobilized carbohydrate bioactivity.

Main Methods:

• Direct self-assembly of thiolated glycosides onto gold surfaces.
• Tethering aminated glycosides to amine-reactive self-assembled monolayers (SAMs).
• Conjugating natural glycans onto divinyl sulfone-activated SAMs.

Main Results:

• Demonstration of three distinct, versatile methods for glyco-SAM preparation.
• Successful immobilization of both natural and chemically modified glycans.
• Establishment of methods for creating functional glycan surfaces for biomimetic studies.

Conclusions:

• The described methods offer robust approaches for preparing functional glyco-SAMs.
• These techniques are essential for advancing glycomics research and understanding glycan functions.
• The development of high-quality glyco-SAMs is key for applications like biosensing and drug discovery.