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Updated: May 27, 2026

Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
[Isolation of alkaloids from Corydalis saxicola by high-speed counter-current chromatography]
Xuan-xuan Cheng1, De-po Yang, Dong-mei Wang
1School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. xuanxuanch@gmail.com
Objective:
To develop a method for the rapid isolation of alkaloids from the crude extract of Corydalis saxicola.
Methods:
High-speed counter-current chromatography (HSCCC) was applied for the separation of alkaloids from Corydalis saxicola, with the biphase solvent systems comprised n-butanol-ethyl acetate-water-formic acid (5: 1: 5: 0.01) and n-butanol-ethyl acetate-methanol-water-formic acid (5: 5: 1:9:0.05).
Results:
About 300 mg of crude extract was isolated by HSCCC, yielding 3.6 mg of scoulerine, 9.2 mg of isocorydine, 5.5 mg of dehydrocheilanthifoline, 7.5 mg of dehydrocavidine, 20.4 mg of palmatine and 20.9 mg of berberine, with purities of 71%, 92%, 85%, 76%, 90%, 97%, respectively.
Conclusion:
It is time-saving and simple to isolate alkaloids from Corydalis saxicola by HSCCC with high yields and purities.
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