Related Experiment Video
Updated: May 27, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Published on: March 20, 2016
Crosstalk between blue-light- and ABA-signaling pathways in stomatal guard cells
Maki Hayashi1, Toshinori Kinoshita
1Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan.
Abstract:
We recently established an immunohistochemical method for the detection of blue light (BL)-induced and phototropin-mediated phosphorylation of plasma-membrane H+-ATPase in stomatal guard cells of Arabidopsis thaliana. This technique makes it possible to detect the phosphorylation/activation status of guard-cell H+-ATPase in the epidermis of a single rosette leaf, without the need to prepare guard-cell protoplasts (GCPs) from a large number of plants. Moreover, it can detect guard-cell responses under more natural and stress-free conditions compared to using GCPs. Taking advantage of these properties, we examined the effect of abscisic acid (ABA) on BL-induced phosphorylation of guard-cell H+-ATPase by using ABA-insensitive mutants. This revealed inhibition of BL-induced phosphorylation of guard-cell H+-ATPase via the early ABA-signaling components PYR/PYL/RCAR-PP2Cs-SnRK2s, which are known to be early ABA-signaling components for a wide range of ABA responses in plants.
Related Concept Videos
Regulation of Transpiration by Stomata
Cell Signaling in Plants
Short-distance Transport of Resources
Adaptations that Reduce Water Loss
Photoreceptors and Plant Responses to Light
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...

