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[Primary detection of biological functions of anti-hGITR(aa27-165);PcAb]
1The Clinical Laboratory of the Affiliated People's Hospital, Zhenjiang 212002, China.
Aim:
To detect the rabbit-derived polyclonal antibodies against extracellular protein segments of human glucocorticoid-inducible tumor necrosis factor receptors (anti-hGITR(aa27-165);PcAb) with regard to its capacity of linkage to natural GITR molecules and the function on CD4(+); T cells.
Methods:
Human peripheral blood mononuclear cells (PBMCs) were isolated and cultured under stimulation; flow cytometry was applied to check the capacity of anti-hGITR(aa27-165);PcAb for linkage to natural GITR molecules on PBMCs; human CD4(+); T cells were isolated by immunological magnetic beads and (3);H-TdR incorporation tests were performed to observe improving-proliferation action of anti-hGITR(aa27-165);PcAb while CD4(+); T cells were cultured with or not with some cytokines.
Results:
Anti-hGITR(aa27-165);PcAb was able to bind GITR molecules with natural conformation in a concentration-dependent way; furthermore, this PcAb could improve the reproduction of CD4(+); T cells.
Conclusion:
The rabbit-derived anti-hGITR(aa27-165);PcAb prepared in our laboratory is capable of linking to natural target molecules and possesses the activation function upon CD4(+); T cells, the further exploration should allow for its applications for diagnosis and treatment of relavent diseases.
